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产品概述
| 名称 | SV-HUC-1 (人输尿管上皮永生化细胞) (STR鉴定正确) |
| 别称 | HUC-1; SV-HUC; SVHUC |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 生长培养基 | Ham's F-12K＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 注意事项 | 该细胞较难消化，注意延长消化时间，消化到细胞收缩变圆，轻拍培养瓶侧边，细胞可以滑落方可终止消化。 |
| 背景描述 | SV-HUC-1细胞是正常输尿管组织用SV40病毒转染建株的。反复用非洲绿猴肾细胞平板测试检测传染性SV40的生成，结果都呈阴性；SV-HUC-1细胞在胁迫(如曝露于化学试剂)下可能激活病毒。 |
| 年龄（性别） | 男；11岁 |
| 组织来源 | 输尿管，输尿管上皮 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | 2 |
| 致瘤性 | No, nude mice. |
| 基因表达情况 | uroepithelial keratins; SV40 T antigen |
| 保藏机构 | ATCC; CRL-9520 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 10,11 D2S1338 17 D3S1358 14,17 D5S818 12,14 D7S820 10,11 D8S1179 14,16 D13S317 12,13 D16S539 11 D18S51 15,16 D19S433 14 D21S11 29,30 FGA 21,22 PentaD 13 PentaE 10,20 TH01 9,9.3 TPOX 8,10 vWA 14 D6S1043 11,13 D12S391 18 D2S441 10,13 -
STR鉴定图
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参考文献
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WD repeat protein 54-mediator of ErbB2-driven cell motility 1 axis promotes bladder cancer tumorigenesis and metastasis and impairs chemosensitivity(2023/01/07)
作者：Xiaosong Wei, Beibei Wang, Zixin Wu
期刊：CANCER LETTERS
DOI：10.1016/j.canlet.2023.216058
影响因子 ：9.700
引用产品：CL-0002 CL-0125 CL-0222 CL-0227 CL-0449A CL-0463 CL-0628 PM150410 PM150710 PM150910 PM151010
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A modified biodegradable mesh ureteral stent for treating ureteral stricture disease(2022/11/17)
作者：Yichun Wang, Xiaohan Ren, Chengjian Ji, Da Zhong, Xiyi Wei, Zheng Zhu, Xuan Zhou, Xi Zhang, Shuai Wang, Chao Qin, Ninghong Song
期刊：Acta Biomaterialia
DOI：10.1016/j.actbio.2022.11.022
影响因子 ：9.300
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RETRACTED: Baicalein inhibits proliferation and migration of bladder cancer cell line T24 by down-regulation of microRNA-106(2018/11/01)
作者：Liangzhen Jiang, Haibin Song, Hongbo Guo
期刊：Biomedicine & Pharmacotherapy
DOI：10.1016/j.biopha.2018.08.107
影响因子 ：7.500
引用产品：CL-0222
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Hypoxia-Induced HIF-1α Expression Promotes Neurogenic Bladder Fibrosis via EMT and Pyroptosis(2022/11/29)
作者：Qi Li, Yifan Hong, Jing Chen, Xiazhu Zhou, Xiaomao Tian, Yihang Yu, Lianju Shen, Chunlan Long, Miao Cai, Shengde Wu, Guanghui Wei
期刊：Cells
影响因子 ：6.000
引用产品：CL-0222
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A novel signature constructed by ferroptosis-associated genes (FAGs) for the prediction of prognosis in bladder urothelial carcinoma (BLCA) and associated with immune infiltration(2021/08/06)
作者：Jiaochen Luan, Tengyue Zeng, Qijie Zhang
期刊：Cancer Cell International
DOI：10.1186/s12935-021-02096-3
影响因子 ：5.800
引用产品：CL-0222
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circ_100984‐miR‐432‐3p axis regulated c‐Jun/YBX‐1/β‐catenin feedback loop promotes bladder cancer progression(2021/02/10)
作者：Liang Tong, Huihui Yang, Wei Xiong
期刊：Cancer Science
影响因子 ：5.700
引用产品：CL-0222
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Ferredoxin 1 is a cuproptosis-key gene responsible for tumor immunity and drug sensitivity: A pan-cancer analysis.(2022/09/21)
作者：Longfei Yang, Yuwei Zhang, Yang Wang
期刊：Frontiers in Pharmacology
影响因子 ：5.600
引用产品：CL-0002 CL-0010 CL-0052 CL-0075 CL-0109 CL-0185 CL-0200 CL-0222 CL-0227 CM-H019 PM150110 PM150210 PM150910
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Immunogenic Cell Death Associated Molecular Patterns and the Dual Role of IL17RA in Interstitial Cystitis/Bladder Pain Syndrome(2023/02/23)
作者：Wei Zhang, Xiaodong Liu, Jiawen Wang
期刊：Biomolecules
影响因子 ：5.500
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CRTAC1 (Cartilage acidic protein 1) inhibits cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) process in bladder cancer by downregulating Yin Yang 1 (YY1) to inactivate the TGF-β pathway(2021/11/24)
作者：Jianghua Yang, Li Fan, Xiaoxing Liao
期刊：Bioengineered
DOI：10.1080/21655979.2021.1974645
影响因子 ：4.900
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The oncogenic role of TFAP2A in bladder urothelial carcinoma via a novel long noncoding RNA TPRG1-AS1/DNMT3A/CRTAC1 axis(2022/11/21)
作者：Jiani He, Changming Dong, Hao Zhang, Yuanjun Jiang, Tao Liu, Xiaojun Man
期刊：CELLULAR SIGNALLING
DOI：10.1016/j.cellsig.2022.110527
影响因子 ：4.800
引用产品：CL-0002 CL-0005 CL-0122 CL-0125 CL-0222 CL-0227 CL-0431
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A pan-cancer analysis of DDR1 in prognostic signature and tumor immunity, drug resistance(2023/04/08)
作者：Longfei Yang, Yuwei Zhang, Yifan Tang
期刊：Scientific Reports
DOI：10.1038/s41598-023-27975-9
影响因子 ：4.600
引用产品：CL-0002 CL-0010 CL-0052 CL-0075 CL-0109 CL-0185 CL-0200 CL-0222 CL-0227 CM-H019 PM150110 PM150210 PM150910
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NCAPH promotes cell proliferation and inhibits cell apoptosis of bladder cancer cells through MEK/ERK signaling pathway( 2022/01/02)
作者：Bo Li, Qian Xiao, Liping Shan
期刊：Cell Cycle
DOI：10.1080/15384101.2021.2021050
影响因子 ：4.300
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Hsa_circ_0008035 Knockdown Inhibits Bladder Cancer Progression through miR-1184/RAP2B Axis(2023/03/23)
作者：Yang Fu, Kun Liu, Xi Jiang
期刊：UROLOGIA INTERNATIONALIS
影响因子 ：1.600
