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产品概述
| 名称 | HFL1 (人胚肺成纤维细胞) (STR鉴定正确) |
| 别称 | HFL-1; HFL 1; Human fetal lung fibroblast 1; HFL |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 生长培养基 | Ham's F-12K＋10%FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 注意事项 | 该细胞为有限传代细胞系，传代次数过高会导致细胞生长特性发生变化，建议到货3代内完成实验。 |
| 年龄（性别） | 男；胎儿 |
| 组织来源 | 肺 |
| 细胞类型 | 有限细胞系 |
| 生物安全等级 | 1 |
| 保藏机构 | ATCC; CCL-153 BCRC; 60299 ECACC; 89071902 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 10,12 D2S1338 17,25 D3S1358 14,17 D5S818 12 D7S820 9,10 D8S1179 12,14 D13S317 11,12 D16S539 9,11 D18S51 18,19 D19S433 11,13 D21S11 27,30 FGA 21,22 PentaD 2.2,9 PentaE 12,20 TH01 7,9 TPOX 6,9 vWA 17 D6S1043 11,18 D12S391 20,21 D2S441 14,15 -
STR鉴定图
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参考文献
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Monitoring and Modulating mtDNA G-Quadruplex Dynamics Reveal Its Close Relationship to Cell Glycolysis(2021/12/06)
作者：Xiucai Chen, Guixue Tang, Wenhua Luo
期刊：Journal Of The American Chemical Society
影响因子 ：16.383
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Templated freezing assembly precisely regulates molecular assembly for free-standing centimeter-scale microtextured nanofilms(2023/02/07)
作者：Junqiang Mao, Huimei Cao, Jie Liu
期刊：Science China-Chemistry
影响因子 ：9.600
引用产品：CL-0106
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Yangqing Chenfei formula attenuates silica-induced pulmonary fibrosis by suppressing activation of fibroblast via regulating PI3K/AKT, JAK/STAT, and Wnt signaling pathway(2022/12/22)
作者：Fan Yang, Runsu Hou, Xinguang Liu
期刊：PHYTOMEDICINE
DOI：10.1016/j.phymed.2022.154622
影响因子 ：7.900
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Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro(2019/03/04)
作者：Deguang Kong, Dan Zhang, Qiuxia Cui
期刊：Biomedicine & Pharmacotherapy
DOI：10.1016/j.biopha.2019.108706
影响因子 ：7.500
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Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis(2021/08/11)
作者：Ai Wei, Qi Gao, Fang Chen
期刊：British Journal Of Pharmacology
影响因子 ：7.300
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p62-Nrf2 Regulatory Loop Mediates the Anti-Pulmonary Fibrosis Effect of Bergenin(2022/02/03)
作者：Qian Zeng, Tingting Zhou, Feiyan Zhao
期刊：Antioxidants
影响因子 ：7.000
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Assessment of 2-allyl-4,5-dimethoxyphenol safety and attractiveness to mature males of Bactrocera dorsalis (Hendel)(2021/08/04)
作者：Shuzhen Deng, Xinyang Li, Ziming Wang
期刊：Ecotoxicology And Environmental Safety
DOI：10.1016/j.ecoenv.2021.112567
影响因子 ：6.800
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Dual inhibitors of ASK1 and PDK1 kinases: Design, synthesis, molecular docking and mechanism studies of N-benzyl pyridine-2-one containing derivatives as anti-fibrotic agents(2022/12/30)
作者：Jia Qin, Meng Cao, Xinlan Hu
期刊：EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1016/j.ejmech.2022.115057
影响因子 ：6.700
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Total flavonoids of Oxytropis falcata Bunge have a positive effect on idiopathic pulmonary fibrosis by inhibiting the TGF-β1/Smad signaling pathway(2021/11/24)
作者：Xinze Li, Xuelin Wang, Yanjun Wang
期刊：Journal of Ethnopharmacology
影响因子 ：5.400
引用产品：CL-0106
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Assessment of attractancy and safeness of (E)-coniferyl alcohol for management of female adults of Oriental fruit fly, Bactrocera dorsalis (Hendel)(2021/11/23)
作者：Huan Liu, Dandan Wang, Lin Wan
期刊：Pest Management Science
DOI：10.1002/ps.6713
影响因子 ：4.100
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Exploration of Clove Bud (Syzygium aromaticum) Essential Oil as a Novel Attractant against Bactrocera dorsalis (Hendel) and Its Safety Evaluation(2022/10/09)
作者：Zhenjie Hu, Jingwei Yang, Zihan Chen
期刊：Insects
影响因子 ：3.000
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Metformin attenuates TGF-β1-induced pulmonary fibrosis through inhibition of transglutaminase 2 and subsequent TGF-β pathways(2020/6/3)
作者：Yubo Wang, Caiyu Lin, Rui Han
期刊：3 BIOTECH
DOI：10.1007/s13205-020-02278-2
影响因子 ：2.800
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Metformin attenuates TGF-β1-induced pulmonary fibrosis through inhibition of transglutaminase 2 and subsequent TGF-β pathways(2020/06/03)
作者：Yubo Wang, Caiyu Lin, Rui Han
期刊：3 Biotech
DOI：10.1007/s13205-020-02278-2
影响因子 ：2.800
引用产品：CL-0106
