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名称 | PC-12 (未分化) (大鼠肾上腺嗜铬细胞瘤细胞(未分化)) (种属鉴定正确) |
背景描述 | PC-12(未分化)细胞是来自能移植的雄性大鼠肾上腺嗜铬细胞瘤。PC-12(未分化)细胞表达神经生长因子(NGF)受体,NGF可诱导产生神经表型。PC-12(未分化)细胞不合成肾上腺素。 |
种属 | 大鼠 |
年龄(性别) | 雄 |
组织来源 | 肾上腺嗜铬细胞瘤 |
生长特性 | 半贴半悬 |
细胞形态 | 不规则形 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肾癌细胞 |
生长培养基 | RPMI-1640+15%HS+5% FBS+1% P/S |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2~3次/周 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
注意事项 | 1、PC-12(未分化)细胞正常生长状态为悬浮聚团,若需要用NGF诱导时,需要使用多聚-L-赖氨酸溶液(货号 PB180523)包被培养瓶后促使细胞贴壁。 2、未分化、低分化和高分化的区别:未分化的PC-12从形态上看是圆形的,聚团生长的漂浮细胞;低分化的成多角形,有较短的突起;高分化的细胞有多个突起,突起数目不等,突触较长,类似神经元轴突。 3、低分化和高分化是在未分化的PC-12基础上诱导产生的,低分化和高分化指的与神经细胞表型的相似程度,高分化更接近神经细胞表型。 |
种属鉴定
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种属鉴定图
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参考文献
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A value-added application of eugenol as acaricidal agent: The mechanism of action and the safety evaluation(2020/12/28)
作者:Xiaofei Shang, Lixia Dai, Chenjie Yang
期刊:Journal of Advanced Research
DOI:10.1016/j.jare.2020.12.010
影响因子 :10.4790
引用产品:CL-0412
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Macroscopic Supramolecular Assembly Strategy to Construct 3D Biocompatible Microenvironments with Site-Selective Cell Adhesion(2021/06/11)
作者:Changyu Wang, Cuiling Lin, Rui Ming
期刊:ACS Applied Materials & Interfaces
影响因子 :10.3830
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Immune-responsive gene 1/itaconate activates nuclear factor erythroid 2-related factor 2 in microglia to protect against spinal cord injury in mice(2022/02/10)
作者:Libin Ni, Jian Xiao, Di Zhang
期刊:Cell Death & Disease
DOI:10.1038/s41419-022-04592-4
影响因子 :9.685
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Low-glucose-sensitive TRPC6 dysfunction drives hypoglycemia-induced cognitive impairment in diabetes(2020/10/18)
作者:Chengkang He, Peng Gao, Yuanting Cui
期刊:Clinical and Translational Medicine
DOI:10.1002/ctm2.205
影响因子 :8.554
引用产品:CL-0412
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Protective effect of plastrum testudinis extract on dopaminergic neurons in a Parkinsons disease model through DNMT1 nuclear translocation and SNCAs methylation(2021/06/18)
作者:Sen Ye, Jun Zhong, Jiapei Huang
期刊:Biomedicine & Pharmacotherapy
DOI:10.1016/j.biopha.2021.111832
影响因子 :7.419
引用产品:CL-0412
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Neuroprotective effects of NDEELNK from sea cucumber ovum against scopolamine-induced PC12 cell damage through enhancing energy metabolism and upregulation of the PKA/BDNF/NGF signaling pathway(2021/06/22)
作者:Yue Zhao, Yifei Dong, Qi Ge
期刊:Food & Function
影响因子 :6.317
引用产品:CL-0412
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Evaluation and structure–activity relationship analysis of antioxidant shrimp peptides(2019/08/16)
作者:Dan Wu, Na Sun, Jie Ding
期刊:Food & Function
影响因子 :6.317
引用产品:CL-0412
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Optimal route of gold nanoclusters administration in mice targeting Parkinson’s disease(2020/02/21)
作者:Jinqi Hu, Guanbin Gao, Meng He
期刊:Nanomedicine
影响因子 :6.096
引用产品:CL-0412
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Neuroprotective Function of a Novel Hexapeptide QMDDQ from Shrimp via Activation of the PKA/CREB/BNDF Signaling Pathway and Its Structure–Activity Relationship(2020/06/02)
作者:Dan Wu, Shuyu Zhang, Na Sun
期刊:Journal Of Agricultural And Food Chemistry
影响因子 :5.895
引用产品:CL-0412
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Egg yolk phosphatidylcholine: Extraction, purification and its potential neuroprotective effect on PC12 cells(2019/03/28)
作者:Jin Chen, Songyi Lin, Na Sun
期刊:Journal of Functional Foods
影响因子 :5.223
引用产品:CL-0412
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Structure-activity relationship and pathway of antioxidant shrimp peptides in a PC12 cell model(2020/05/04)
作者:Dan Wu, Mengqi Li, Jie Ding
期刊:Journal of Functional Foods
影响因子 :5.223
引用产品:CL-0412
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Adipose Mesenchymal Stem Cell-Derived Exosomes Enhance PC12 Cell Function through the Activation of the PI3K/AKT Pathway(2021/10/15)
作者:Yong Xie, Yangping Chen, Yuyuan Zhu
期刊:Stem Cells International
影响因子 :5.131
引用产品:CL-0412