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产品概述
| 名称 | LoVo (人结肠癌细胞) (STR鉴定正确) |
| 别称 | LOVO |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 生长培养基 | Ham's F-12K＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 背景描述 | LoVo细胞是于1971年从诊断为结肠腺癌的56岁男性白人的一个转移到左锁骨上区的肿瘤结节建系而来。癌基因检测表明，LoVo细胞C-myc、K-ras、H-ras、N-ras、Myb、sis和fos的表达呈阳性；癌基因N-myc的表达未做检测。LoVo细胞在裸鼠中能成瘤，与107细胞联合皮下接种5只裸鼠21天后全部成瘤。LoVo表达肿瘤特异的核基质蛋白蛋白CC-3和CC-4。 |
| 年龄（性别） | 男；56岁 |
| 组织来源 | 直结肠腺癌，转移位置：左锁骨上区 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | 1 |
| 倍增时间 | ~34-52 hours |
| 致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
| 抗原表达情况 | HLA A11, B15, B17, Cw1, Cw3; Blood Type B |
| 基因表达情况 | carcinoembryonic antigen (CEA) 908 ng/10^6 cells/10 days. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3. |
| 保藏机构 | ATCC; CCL-229 BCRC; 60148 BCRJ; 0332 CCLV; CCLV-RIE 1151 DSMZ; ACC-350 ECACC; 87060101 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 10,11,13,14 D2S1338 17,18 D3S1358 14,17,18 D5S818 11,13 D7S820 9.3,10,11 D8S1179 10 D13S317 8,11 D16S539 9,12 D18S51 13,18 D19S433 14,15 D21S11 29,31.2 FGA 18,20 PentaD 10,14 PentaE 10,16 TH01 9.300000000000001 TPOX 8,9 vWA 17,18 D6S1043 11,17 D12S391 20,22,23 D2S441 13,14 -
STR鉴定图
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参考文献
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Structural elucidation of an acidic polysaccharide from Citrus grandis ‘Tomentosa’ and its anti-proliferative effects on LOVO and SW620 cells(2019/10/01)
作者：Ruiyi Fan, Yuming Xie, Congyi Zhu
期刊：International Journal of Biological Macromolecules
DOI：10.1016/j.ijbiomac.2019.07.117
影响因子 ：8.200
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Antitumor efficacy of a recombinant EGFR-targeted fusion protein conjugate that induces telomere shortening and telomerase downregulation(2022/11/23)
作者：Hong-yu Tao, Shi-ming He, Chun-yan Zhao, Ying Wang, Wei-jin Sheng, Yong-su Zhen
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2022.11.225
影响因子 ：8.200
引用产品：CL-0144
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Anti-colon cancer effects of Spirulina polysaccharide and its mechanism based on 3D models(2022/12/26)
作者：Yingfang Lu, Zexin Chen, Qianmin Lin
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2022.12.244
影响因子 ：8.200
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c-Met-Targeting 19F MRI Nanoparticles with Ultralong Tumor Retention for Precisely Detecting Small or Ill-Defined Colorectal Liver Metastases(2023/04/25)
作者：Daoshuang Li, Jie Yang, Zuoyu Xu
期刊：International Journal of Nanomedicine
影响因子 ：8.000
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Discovery of a Potent, Cooperative, and Selective SOS1 PROTAC ZZ151 with In Vivo Antitumor Efficacy in KRAS-Mutant Cancers(2023/03/10)
作者：Zehui Zhou, Guizhen Zhou, Chuan Zhou
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.3c00075
影响因子 ：7.300
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Circ-RNF121 regulates tumor progression and glucose metabolism by miR-1224-5p/FOXM1 axis in colorectal cancer(2021/11/06)
作者：Zhipeng Jiang, Hao Hu, Wenli Hu
期刊：Cancer Cell International
DOI：10.1186/s12935-021-02290-3
影响因子 ：5.800
引用产品：164210 164210-50 CL-0096 CL-0144 CL-0223B CL-0225B PB180120 PM150110 PM150210
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Circ_0011385 knockdown inhibits cell proliferation, migration and invasion, whereas promotes cell apoptosis by regulating miR-330-3p/MYO6 axis in colorectal cancer(2022/01/26)
作者：Jing Wang, Shaobo Ke, Yi Gong
期刊：Biomedical Journal
影响因子 ：5.500
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Loss of Tumor Suppressor C9orf9 Promotes Metastasis in Colorectal Cancer(2023/02/07)
作者：Erfei Chen, Fangfang Yang, Qiqi Li
期刊：Biomolecules
影响因子 ：5.500
引用产品：CL-0144
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CircFAM120B Blocks the Development of Colorectal Cancer by Activating TGF-Beta Receptor II Expression via Targeting miR-645(2021/07/26)
作者：You Yu, Xiao Lei
期刊：Frontiers in Cell and Developmental Biology
影响因子 ：5.500
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Long non-coding RNA HOXA-AS3 facilitates the malignancy in colorectal cancer by miR-4319/SPNS2 axis(2021/10/21)
作者：Yang Jiang, Xiaoyu Yu, Huixin Sun
期刊：Journal Of Physiology And Biochemistry
DOI：10.1007/s13105-021-00832-x
影响因子 ：5.080
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MicroRNA-934 facilitates cell proliferation, migration, invasion and angiogenesis in colorectal cancer by targeting B-cell translocation gene 2(2021/10/16)
作者：Bo Li, Xianyi Liu, Guogang Wu
期刊：Bioengineered
DOI：10.1080/21655979.2021.1996505.
影响因子 ：4.900
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Blocking NFATc3 ameliorates azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer in mice via the inhibition of inflammatory responses and epithelial-mesenchymal transition(2020/07/10)
作者：Yan Lin, Moussa Harouna Koumba, Suxuan Qu
期刊：Cellular Signalling
DOI：10.1016/j.cellsig.2020.109707
影响因子 ：4.800
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Hsa-miR-425-5p promotes tumor growth and metastasis by activating the CTNND1-mediated β-catenin pathway and EMT in colorectal cancer(2020/06/28)
作者：Dingsheng Liu, Hong Zhang, Mingming Cui
期刊：Cell Cycle
DOI：10.1080/15384101.2020.1783058
影响因子 ：4.300
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Circ_0056618 enhances PRRG4 expression by competitively binding to miR-411-5p to promote the malignant progression of colorectal cancer(2022/08/02)
作者：Bo Zhang, Wenbin Cao, Yang Liu
期刊：MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI：10.1007/s11010-022-04525-x
影响因子 ：4.300
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miR-145-5p restrained cell growth, invasion, migration and tumorigenesis via modulating RHBDD1 in colorectal cancer via the EGFR-associated signaling pathway(2019/11/03)
作者：Ying Niu, Jinping Zhang, Yalin Tong
期刊：International Journal Of Biochemistry & Cell Biology
DOI：10.1016/j.biocel.2019.105641
影响因子 ：4.000
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miR-140-3p inhibits colorectal cancer progression and its liver metastasis by targeting BCL9 and BCL2(2021/04/10)
作者：Dingsheng Liu, Chunsheng Chen, Mingming Cui
期刊：Cancer Medicine
影响因子 ：4.000
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P300 increases CSNK2A1 expression which accelerates colorectal cancer progression through activation of the PI3K-AKT-mTOR axis(2023/06/28)
作者：Jilong Liu
期刊：EXPERIMENTAL CELL RESEARCH
DOI：10.1016/j.yexcr.2023.113694
影响因子 ：3.700
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The bromodomain and extra-terminal domain inhibitor JQ1 synergistically sensitizes human colorectal cancer cells to topoisomerase I inhibitors through repression of Mre11-mediated DNA repair pathway(2020/09/26)
作者：Linping Lei, Xuqin Xie, Long He
期刊：Investigational New Drugs
DOI：10.1007/s10637-020-01014-0
影响因子 ：3.400
引用产品：CL-0096 CL-0118 CL-0144 CL-0196 CL-0223A CL-0223B CL-0225A CL-0225B
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Anti-cancer effects of Porphyra haitanensis polysaccharides on human colon cancer cells via cell cycle arrest and apoptosis without causing adverse effects in vitro(2020/8/11)
作者：WanZi Yao, Suresh Veeraperumal, HuaMai Qiu
期刊：3 BIOTECH
DOI：10.1007/s13205-020-02379-y
影响因子 ：2.800
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Cullin-4B promotes cell proliferation and invasion through inactivation of p53 signaling pathway in colorectal cancer(2021/6/8)
作者：Min Zhong, Ling Zhou, Jianping Zou
期刊：Pathology Research And Practice
影响因子 ：2.800
引用产品：CL-0144
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microRNA-4429-5p suppresses the malignant development of colon cancer by targeting matrix metalloproteinase 16(2021/08/26)
作者：Wei Li, Zhe Song, Nan Jia
期刊：In Vitro Cellular & Developmental Biology-Animal
DOI：10.1007/s11626-021-00603-4
影响因子 ：2.723
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circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis(2021/8/10)
作者：HaiLin Xiong, XiaoHua Zhong, XiaoHong Guo
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.700
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Circ_0000467 Exerts an Oncogenic Role in Colorectal Cancer via miR-330-5p-Dependent Regulation of TYRO3(2022/01/18)
作者：Yubao Huang, Zhiyu Chen, Xiong Zhou, Hai Huang
期刊：Biochemical Genetics
DOI：10.1007/s10528-021-10171-7
影响因子 ：2.400
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Trefoil factor 3 knock-down prevents autophagy-related gene 12 elevation in colon adenocarcinoma(2019/07/30)
作者：Zhuo Zhang
期刊：Journal of Histotechnology
DOI：10.1080/01478885.2019.1633088
影响因子 ：1.100
引用产品：CL-0144
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Effects of microRNA95 targeting FOXD1 gene on radiosensitivity of colorectal cancer loVo cells and its mechanism(2020 /03)
作者：Wanling Liang, Tianbao Xiao, Feng Yuan
期刊：中国应用生理学杂志
DOI：10.12047/j.cjap.5864.2020.033
影响因子 ：0.950
引用产品：CL-0144
