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产品概述
名称 | SW620 (人结肠癌细胞) (STR鉴定正确) |
别称 | SW-620; SW 620; SW.620 |
种属 | 人类 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,100%, 温度:37℃
|
培养方案B(可选) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 1.该细胞推荐使用Leibovitz's L-15培养基进行培养,Leibovitz's L-15不可以通入二氧化碳,会产生细胞毒性;2.如您没有无二氧化碳的培养箱,可使用DMEM替代Leibovitz's L-15,使用DMEM培养基时即可正常通入5%二氧化碳;3.配套专用培养基默认Leibovitz's L-15配置,如需DMEM配方,请联系销售下单备注更改。 |
背景描述 | SW620细胞是从一个51岁男性白人组织中分离得到的,由A·Leibovitz等从一个淋巴结建株。SW620细胞主要由无绒毛的小园球细胞和双极细胞组成;SW620细胞仅合成少量癌胚抗原(CEA),在裸鼠中有高度的致瘤性。 |
年龄(性别) | 男性;51岁 |
组织来源 | 结直肠腺癌,来自转移淋巴结 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~20-26 hours |
致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
抗原表达情况 | Blood Type A; Rh+ |
基因表达情况 | Carcinoembryonic antigen (CEA) 0.15 ng/10^6 cells/10 days; transforming growth factor alpha; matrilysin. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative. The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. |
保藏机构 | ATCC; CCL-227 ECACC; 87051203 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 13,14 D2S1338 17,24 D3S1358 16 D5S818 13 D7S820 8,9 D8S1179 13 D13S317 12 D16S539 9,13 D18S51 13 D19S433 13 D21S11 30,30.2 FGA 24 PentaD 9,15 PentaE 10 TH01 8 TPOX 11 vWA 16 D6S1043 11,12 D12S391 17 D2S441 10,15 -
STR鉴定图
参考文献
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Circ_0011385 knockdown inhibits cell proliferation, migration and invasion, whereas promotes cell apoptosis by regulating miR-330-3p/MYO6 axis in colorectal cancer(2022/01/26)
作者:Jing Wang, Shaobo Ke, Yi Gong
期刊:Biomedical Journal
影响因子 :5.500
引用产品:164210 164210-50 CL-0096 CL-0144 CL-0223 CL-0225 PM150110 PM150210
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Raltitrexed as a synergistic hyperthermia chemotherapy drug screened in patient-derived colorectal cancer organoids(2021/8/15)
作者:Lisi Zeng, Quanxing Liao, Haoran Zhao
期刊:Cancer Biology & Medicine
DOI:10.20892/j.issn.2095-3941.2020.0566
影响因子 :5.500
-
Notum palmitoleoyl-protein carboxylesterase regulates Fas cell surface death receptor-mediated apoptosis via the Wnt signaling pathway in colon adenocarcinoma(2021/8/17)
作者:Hua Gong, Qiang Niu, Yi Zhou
期刊:Bioengineered
DOI:10.1080/21655979.2021.1961657
影响因子 :4.900
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Circ_0056618 enhances PRRG4 expression by competitively binding to miR-411-5p to promote the malignant progression of colorectal cancer(2022/08/02)
作者:Bo Zhang, Wenbin Cao, Yang Liu
期刊:MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI:10.1007/s11010-022-04525-x
影响因子 :4.300
引用产品:164210 164210-50 CL-0096 CL-0144 CL-0223 CL-0225 PM150710 PM151010
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MicroRNA‑129 inhibits colorectal cancer cell proliferation, invasion and epithelial‑to‑mesenchymal transition by targeting SOX4(2021/03/24)
作者:Zhiping Chen, Tianyu Zhong, Jinghua Zhong
期刊:Oncology Reports
影响因子 :4.200
-
MicroRNA‑129 inhibits colorectal cancer cell proliferation, invasion and epithelial‑to‑mesenchymal transition by targeting SOX4(2021/03/24)
作者:Zhiping Chen, Tianyu Zhong, Jinghua Zhong
期刊:Oncol Rep
影响因子 :4.136
-
NDRG4 sensitizes CRC cells to 5-FU by upregulating DDIT3 expression(2021/9/13)
作者:Ruikai Li, Chenxiang He, Liangliang Shen
期刊:Oncology Letters
影响因子 :2.900
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microRNA-4429-5p suppresses the malignant development of colon cancer by targeting matrix metalloproteinase 16(2021/08/26)
作者:Wei Li, Zhe Song, Nan Jia
期刊:In Vitro Cellular & Developmental Biology-Animal
DOI:10.1007/s11626-021-00603-4
影响因子 :2.723
引用产品:CL-0050 CL-0096 CL-0144 CL-0223 CL-0225 PM150710 PM150910 PM151010