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产品概述
名称 | SW620 (人结肠癌细胞) (STR鉴定正确) |
别称 | SW-620; SW 620; SW.620 |
种属 | 人 |
年龄(性别) | 男;51岁 |
组织来源 | 结直肠腺癌,来自转移淋巴结 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
背景描述 | SW620细胞是从一个51岁男性白人组织中分离得到的,由A·Leibovitz等从一个淋巴结建株。SW620细胞主要由无绒毛的小园球细胞和双极细胞组成;SW620细胞仅合成少量癌胚抗原(CEA),在裸鼠中有高度的致瘤性。 |
生物安全等级 | 1 |
生长培养基 | Leibovitz's L-15(PM151010)+10% FBS(164210-500)+1% P/S(PB180120) |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
倍增时间 | ~20-26 hours |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,100% 温度:37℃ |
致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
抗原表达情况 | Blood Type A; Rh+ |
基因表达情况 | Carcinoembryonic antigen (CEA) 0.15 ng/10^6 cells/10 days; transforming growth factor alpha; matrilysin. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative. The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. |
保藏机构 | ATCC; CCL-227 ECACC; 87051203 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 13,14 D2S1338 17,24 D3S1358 16 D5S818 13 D7S820 8,9 D8S1179 13 D13S317 12 D16S539 9,13 D18S51 13 D19S433 13 D21S11 30,30.2 FGA 24 PentaD 9,15 PentaE 10 TH01 8 TPOX 11 vWA 16 D1S1656 13,14 D6S1043 11,12 D12S391 17 -
STR鉴定图
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参考文献
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Structural elucidation of an acidic polysaccharide from Citrus grandis ‘Tomentosa’ and its anti-proliferative effects on LOVO and SW620 cells(2019.10.01)
作者:Ruiyi Fan, Yuming Xie, Congyi Zhu
期刊:International Journal of Biological Macromolecules
DOI:10.1016/j.ijbiomac.2019.07.117
影响因子 :5.162
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A 3-circular RNA signature as a noninvasive biomarker for diagnosis of colorectal cancer(2019.11.04)
作者:Dao-xiong Ye, Si-si Wang, Ying Huang
期刊:Cancer Cell International
影响因子 :4.175
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Bone Morphogenetic Protein 1 Targeting COL1A1 and COL1A2 to Regulate the Epithelial-Mesenchymal Transition Process of Colon Cancer SW620 Cells(2020.03)
作者:Zhu, Xijia; Luo, Xishun; Jiang, Shiyu; Wang, Haipeng
期刊:Journal of Nanoscience and Nanotechnology
影响因子 :1.134
引用产品:CL-0225