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产品概述
名称 | RKO (人结肠腺癌细胞) (STR鉴定正确) |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
生长培养基 | MEM(含NEAA)+10% FBS+1% P/S |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
注意事项 | 该细胞对温度变化敏感,培养时使用的培养基需预热,温度较低的培养基使用会导致变圆收缩;传代后到细胞完全伸展开需要72h以上。 |
背景描述 | RKO细胞是一个低分化的结肠癌细胞系。RKO细胞含有野生型p53,但缺乏人甲状腺受体核受体(h-TRβ1)。RKO细胞的p53蛋白的水平高于RKO-E6细胞,RKO细胞是RKO-E6细胞和RKO-AS45-1细胞的亲本细胞系。RKO细胞在裸鼠中成瘤,且在软琼脂中形成集落。 |
组织来源 | 结肠癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | 1 |
倍增时间 | ~21 hours |
致瘤性 | Yes, in nude mice.Yes, in soft agar. |
受体表达情况 | urokinase receptor (u-PAR) |
保藏机构 | ATCC; CRL-2577 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 8,10 D2S1338 16 D3S1358 16,19 D5S818 11,13 D7S820 8,10 D8S1179 9,13,14 D13S317 8,11 D16S539 12,13 D18S51 11,12 D19S433 14 D21S11 27,30 FGA 20,21,22,23 PentaD 10,11 PentaE 11,13 TH01 6,10 TPOX 11 vWA 16,22 D6S1043 14.1,19 D12S391 15,20 D2S441 11 -
STR鉴定图
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参考文献
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Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac4C acetylation of KIF23 mRNA(2022/12/15)
作者:Chi Jin, Tuo Wang, Dongsheng Zhang
期刊:JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI:10.1186/s13046-022-02551-7
影响因子 :11.300
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Suppression of Tumor or Host Intrinsic CMTM6 Drives Antitumor Cytotoxicity in a PD-L1–Independent Manner(2023/02/03)
作者:Yiru Long, Runqiu Chen, Xiaolu Yu
期刊:Cancer Immunology Research
DOI:10.1158/2326-6066.CIR-22-0439
影响因子 :10.100
引用产品:CL-0005 CL-0007 CL-0019 CL-0071 CL-0105 CL-0129 CL-0140 CL-0196 CL-0246 CL-0319
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PD-1-siRNA Delivered by Attenuated Salmonella Enhances the Antitumor Effect of Chloroquine in Colon Cancer(2021/07/06)
作者:Shuya Lu, Jianhui Gao, Huijie Jia
期刊:Frontiers in Immunology
影响因子 :7.300
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Attenuated Salmonella-delivered PD-1 siRNA enhances the antitumor effects of EZH2 inhibitors in colorectal cancer(2023/09/12)
作者:Mengmeng Lv, Sheng Guo, Xinyu Zhang
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2023.110918
影响因子 :5.600
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Notum palmitoleoyl-protein carboxylesterase regulates Fas cell surface death receptor-mediated apoptosis via the Wnt signaling pathway in colon adenocarcinoma(2021/8/17)
作者:Hua Gong, Qiang Niu, Yi Zhou
期刊:Bioengineered
DOI:10.1080/21655979.2021.1961657
影响因子 :4.900
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Fluorescent Quinolinium Derivative as Novel Mitochondria Probe and Function Modulator by Targeting Mitochondrial RNA(2023/03/16)
作者:Bozheng Wang, Yingchen Zhou, Yuwei Lin
期刊:MOLECULES
影响因子 :4.600
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Variation of Saponins in Sanguisorba officinalis L. before and after Processing (Paozhi) and Its Effects on Colon Cancer Cells In Vitro(2022/12/19)
作者:Zhengyang Wang, Chunjuan Yang, Lihong Wu
期刊:MOLECULES
影响因子 :4.600
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EGCG Enhances the Chemosensitivity of Colorectal Cancer to Irinotecan through GRP78-MediatedEndoplasmic Reticulum Stress.(2022/09/13)
作者:Wenbing Wu, Hui Gou, Bin Xiang
期刊:Journal of Oncology
影响因子 :4.501
引用产品:CL-0196
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Three New Compounds, Licopyranol A–C, Together with Eighteen Known Compounds Isolated from Glycyrrhiza glabra L. and Their Antitumor Activities(2022/09/23)
作者:Shanshan Wang, Jameel Hizam Alafifi, Qin Chen
期刊:Metabolites
影响因子 :4.100
引用产品:CL-0196
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CILP2: A prognostic biomarker associated with immune infiltration in colorectal cancer.(2023/04/20)
作者:Xueli Wang, Yu Zhang, Niping Song
期刊:Heliyon
DOI:10.1016/j.heliyon.2023.e15535
影响因子 :4.000
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The bromodomain and extra-terminal domain inhibitor JQ1 synergistically sensitizes human colorectal cancer cells to topoisomerase I inhibitors through repression of Mre11-mediated DNA repair pathway(2020/09/26)
作者:Linping Lei, Xuqin Xie, Long He
期刊:Investigational New Drugs
DOI:10.1007/s10637-020-01014-0
影响因子 :3.400
引用产品:CL-0096 CL-0118 CL-0144 CL-0196 CL-0223A CL-0223B CL-0225A CL-0225B