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产品概述
名称 | Jurkat, Clone E6-1 (人T淋巴细胞白血病细胞) (STR鉴定正确) |
别称 | JurkatE6-1; Jurkat E6-1; Jurkat, Clone E6-1; Jurkat Clone E6-1; Jurkat (clone E6-1); JURKAT E-6.1; JURKAT E-61; Jurkat-E6; Jurkat E6; J.E6-1; E6-1 |
种属 | 人 |
生长特性 | 悬浮细胞 |
细胞形态 | 淋巴细胞样 |
生长培养基 | RPMI-1640+10% FBS+1% P/S |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5% 温度:37℃ |
推荐传代比例 | 3×10^5-5×10^5cells/mL |
推荐换液频率 | 2~3次/周 |
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
背景描述 | Jurkat, Clone E6-1细胞是Jurkat-FHCRC细胞株(Jurkat细胞株的衍生)的一个克隆。Jurkat, Clone E6-1细胞来源于一个14岁男孩的外周血,经佛波酯和外源凝集素或抗T3单克隆抗体(两种类型的诱导都需要)诱导后可产生大量IL-2。 |
年龄(性别) | 男 |
组织来源 | T淋巴细胞;急性T细胞白血病 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 白血病细胞 |
生物安全等级 | 1 |
倍增时间 | ~24-48 hours |
受体表达情况 | T cell antigen receptor, expressed |
抗原表达情况 | CD3; Homo sapiens, expressed |
基因表达情况 | interleukin-2 (interleukin 2, IL-2), CD3; Homo sapiens, expressed |
保藏机构 | ATCC; TIB-152 BCRC; 60424 BCRJ; 0125 ECACC; 88042803 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 19,23 D3S1358 15 D5S818 9 D7S820 8,12 D8S1179 13,14 D13S317 8,12 D16S539 11 D18S51 13,21 D19S433 14,15.2 D21S11 31.2,33.2 FGA 20,21 PentaD 11,13 PentaE 10,12 TH01 6,9.3 TPOX 8,10 vWA 18 D6S1043 11 D12S391 23 D2S441 14,15 -
STR鉴定图
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参考文献
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Single-cell analysis of peripheral blood from high-altitude pulmonary hypertension patients identifies a distinct monocyte phenotype(2023/03/31)
作者:Xinhua Wu, Yangyang He, Zhangrong Chen
期刊:Nature Communications
DOI:10.1038/s41467-023-37527-4
影响因子 :16.600
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An IgD-Fc-Ig fusion protein restrains the activation of T and B cells by inhibiting IgD-IgDR-Lck signaling in rheumatoid arthritis(2021/04/16)
作者:Xiaoxi Hu, Aijun Zhang, Wenwen Pan
期刊:Acta Pharmacologica Sinica
DOI:10.1038/s41401-021-00665-w
影响因子 :8.200
引用产品:CL-0129
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Identification of the regulatory role of lncRNA HCG18 in myasthenia gravis by integrated bioinformatics and experimental analyses(2021/11/18)
作者:Shuang Li, Xu Wang, Tianfeng Wang
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-021-03138-0
影响因子 :7.400
引用产品:CL-0129
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Therapeutic exosomal vaccine for enhanced cancer immunotherapy by mediating tumor microenvironment( 2021/12/17)
作者:Fangfang Lv, Huifang Liu, Gaoqian Zhao
期刊:iScience
DOI:10.1016/j.isci.2021.103639
影响因子 :5.800
引用产品:CL-0129
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ASCT2-mediated glutamine uptake of epithelial cells facilitates CCL5-induced T cell infiltration via ROS-STAT3 pathway in oral lichen planus(2023/04/26)
作者:Jingyi Lu, Zhangci Su, Wei Li
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2023.110216
影响因子 :5.600
引用产品:CL-0129
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Cosmc transfection decreases malignant behavior of Tn+ cells and enhances sensitivity to apoptosis when induced by Apo2L/TRAIL via alteration of O-glycan structure(2021/10/13)
作者:Ruisong Ding, Xingyou Hu, Wen Hu
期刊:Aging-US
影响因子 :5.200
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CXCL9, 10, 11/CXCR3 Axis Contributes to the Progress of Primary Sjogren’s Syndrome by Activating GRK2 to Promote T Lymphocyte Migration(2023/02/18)
作者:Jing Zhang, Xiao Zhang, Xingjie Shi
期刊:INFLAMMATION
DOI:10.1007/s10753-023-01791-9
影响因子 :5.100
引用产品:CL-0129
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Glyoxalase 1 inhibitor BBGC suppresses the progression of chronic lymphocytic leukemia and promotes the efficacy of Palbociclib(2023/02/02)
作者:Jiameng Tang, Jialing Zhong, Zheng Yang
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2023.01.034
影响因子 :3.100
引用产品:CL-0129
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Circ_0000745 regulates NOTCH1-mediated cell proliferation and apoptosis in pediatric T-cell acute lymphoblastic leukemia through adsorbing miR-193b-3p(2021/11/09)
作者:Huanhuan Feng, Fei Li, Ping Tang
期刊:Hematology
DOI:10.1080/16078454.2021.1997197
影响因子 :1.900
引用产品:164210 164210-50 CL-0058 CL-0129 CP-H158 PB180120 PM150110