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产品概述
| 名称 | HCT-8 [HRT-18] (人回盲肠癌细胞) (STR鉴定正确) |
| 别称 | HCT-8; HCT8 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 生长培养基 | RPMI-1640＋10% FBS＋1% P/S |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5% 温度：37℃ |
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2~3次/周 |
| 背景描述 | HCT-8 [HRT-18]细胞与HRT-18细胞是一样的；角蛋白免疫过氧化物酶染色阳性。 |
| 年龄（性别） | 男；67岁 |
| 组织来源 | 结肠；回盲肠结直肠腺癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | 1 |
| 致瘤性 | Yes, in nude mice(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^8 cells). |
| 基因表达情况 | carcinoembryonic antigen (CEA) 0.5 ng/10^6 cells/10 days; alkaline phosphatase. The cells are positive for keratin by immunoperoxidase staining. |
| 保藏机构 | ECACC; 90032006 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 17,25 D3S1358 17 D5S818 13 D7S820 10,11.3 D8S1179 15 D13S317 8,11 D16S539 12,13 D18S51 11,17 D19S433 14,16 D21S11 29,32.2 FGA 22 PentaD 9,14 PentaE 7,14 TH01 7,9.3 TPOX 8,11 vWA 18,19 D6S1043 11,13 D12S391 19,22 D2S441 13,15 -
STR鉴定图
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参考文献
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The feedback loop of ANKHD1/lncRNA MALAT1/YAP1 strengthens the radioresistance of CRC by activating YAP1/AKT signaling(2022/02/02)
作者：Pingan Yao, Yong Wu , Kui Zhao
期刊：Cell Death & Disease
DOI：10.1038/s41419-022-04554-w
影响因子 ：9.000
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The Active Fraction of Polyrhachis vicina Roger (AFPR) activates ERK to cause necroptosis in colorectal cancer(2023/04/12)
作者：Dongmei Li, Fucui Zhu, Jie Wei
期刊：JOURNAL OF ETHNOPHARMACOLOGY
影响因子 ：5.400
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Aureusidin derivative CNQX inhibits chronic colitis inflammation and mucosal barrier damage by targeting myeloid differentiation 2 protein(2021/07/11)
作者：Yi Yang, Yongjia Sheng, Jin Wang
期刊：Journal Of Cellular And Molecular Medicine
影响因子 ：5.300
引用产品：CL-0098
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Site-directed Late-Stage Diversification of Macrocyclic Nannocystins Facilitating Anticancer SAR and Mode of Action Studies(2022/12/22)
作者：Han Zhang, Yunfeng Tian, Xiaoya Yuan
期刊：RSC Medicinal Chemistry
影响因子 ：4.100
