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产品概述
产品信息
| 名称 | OE19 (人食管癌细胞) (STR鉴定正确) |
| 别称 | OE-19; JROECL 19; JROECL19; OEC19 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3天/次 |
参考资料(来源文献)
| 背景描述 | OE19, also known as JROECL19, was originally one of five permanent human oesophageal adenocarcinoma (EAC) cell lines established in 1993. It is from a stage III tumour of a 72 year old male patient1 and deposited in ECACC by Drs J C Rockett/A Morris, Department of Biological Sciences, University of Warwick and Dr S J Darnton, Birmingham Heartlands Hospital. Boonstra et al. discovered that cell lines SEG-1, BIC-1, and SK-GT-5 are not EAC cell lines but large cell lung cancer cell line H460, colorectal adenocarcinoma cell line SW620, and gastric fundus carcinoma cell line SKGT-2, respectively. However, they confirmed that cell lines FLO-1, KYAE-1, SK-GT-4, OE19, OE33, OACP4C, OACM5.1, ESO26, and ESO51 are derived from human EACs2 . All nine of these verified EAC cell lines, together with their genotyping information, have been deposited with ECACC. |
| 年龄（性别） | 男性；72岁 |
| 组织来源 | 食管癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 食管癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~50-60 hours (DSMZ=ACC-700) |
| 致瘤性 | 裸小鼠致癌 |
| 保藏机构 | ECACC; 96071721 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11,13 D2S1338 18,19 D3S1358 15,18 D5S818 11,14 D7S820 8 D8S1179 13,15 D13S317 9,11 D16S539 12,13 D18S51 12 D19S433 13 D21S11 30 FGA 23,26 PentaD 9 PentaE 5,8 TH01 8,9 TPOX 8 vWA 16,17,18 D6S1043 19 D12S391 20 D2S441 14,15 -
STR鉴定图
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![NCI-H146 [H146] (人小细胞肺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0693-1.jpg)
