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| 名称 | DMS 53 (人肺癌细胞) (STR鉴定正确) |
| 别称 | DMS-53; DMS53; Darmouth Medical School 53 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:3 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | DMS 53 is a cell line exhibiting epithelial morphology that was isolated in 1974 from the lung of a 54-year-old, White male patient with carcinoma. |
| 年龄（性别） | 男性；54岁 |
| 组织来源 | 肺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | 5.7 days (PubMed=6266631); 4.9 days (PubMed=2986244) |
| 致瘤性 | Yes; Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10^7 cells. |
| 受体表达情况 | Bombesin, expressed; epidermal growth factor (EGF), expressed; transforming growth factor beta (TGF beta), expressed; acetylcholine, expressed |
| 抗原表达情况 | Leu 7; My23 |
| 基因表达情况 | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; human chorionic gonadotropin (hCG); glucagon; growth hormone; 17 beta estradiol; thyroid releasing hormone; oxytocin - neurophysin (OT-NP); parathormone; somatostatin-like immunoreactivity (SRIF) |
| 保藏机构 | AddexBio; C0016027/4988 ATCC; CRL-2062 ECACC; 95062823 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 25 D3S1358 15 D5S818 10,11 D7S820 8,11 D8S1179 12 D13S317 10 D16S539 12,13 D18S51 15,16 D19S433 13 D21S11 33.2 FGA 20 PentaD 10 PentaE 13 TH01 8,9.3 TPOX 12 vWA 15,17 D6S1043 11,12 D12S391 19,23 D2S441 14 -
STR鉴定图
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